Metabolic Alteration of MCF-7 Cells upon Indirect Exposure to E. coli Secretome: A Model of Studying the Microbiota Effect on Human Breast Tissue

According to studies, the microbiome may contribute emergence and spread of breast cancer. E. coli is one Enterobacteriaceae family recently found be present as part tissue microbiota. In this study, we focused on effect secretome free cells MCF-7 metabolism. Liquid chromatography–mass spectrometry (LC-MS) metabolomics was used study its role in intra- extracellular metabolites. A comparison made between secretome-exposed unexposed controls. Our analysis revealed significant alterations 31 intracellular 55 metabolites following exposure. Several metabolic pathways, including lactate, aminoacyl-tRNA biosynthesis, purine metabolism, energy were dysregulated upon can alter cancer cells’ metabolism through which disrupts key pathways cells. These microbial from hold promise biomarkers drug resistance or innovative approaches for treatment, either standalone therapies combination with other medicines.